Roth’s team named their new technology Barcode Fusion Genetics-Yeast Two Hybrid (BFG-Y2H). The Y2H system is rigged so that only cells in which bait and prey proteins stick together can survive and this allows scientists to see which proteins associate with which other proteins. In a widely-used method called Yeast Two Hybrid (Y2H), yeast cells carrying a ‘bait’ protein are mated with yeast cells carrying a ‘prey’ protein. By combining barcodes inside cells, we can dramatically increase the number of experiments we can combine together in a single test tube.“ “ However it has been one-dimensional, in the sense that we only got to read one experiment per barcode. “ Using DNA barcodes for multiplex experiments has been an extremely powerful technology,” said Frederick (Fritz) Roth, senior author on the study who is cross-appointed at Donnelly Centre and Lunenfeld-Tanenbaum Research Institute, and is also a Canada Excellence Research Chair and Senior Fellow of the Canadian Institute for Advanced Research. The new technology results in a 10-fold increased rate of discovery for the same price. Allowing barcodes to fuse together inside cells means scientists can now break that barrier. However, the number of experiments that can be combined in the same tube has been limited to the number of barcoded cell types. This has been further enabled by next-generation DNA sequencing which can efficiently count barcodes and ‘read out’ the results. In recent years, DNA barcoding has enabled scientists to carry out highly parallel experiments, in which many different types of cells can be tested in the same tube. The paper will be published today in the journal Molecular Systems Biology. A team of researchers at University of Toronto’s Donnelly Centre and Sinai Health System’s Lunenfeld-Tanenbaum Research Institute (LTRI) has developed a new technology that can stitch together DNA barcodes inside a cell to simultaneously search amongst millions of protein pairs for protein interactions.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |